The contact is completed at the equator of early gastrulae and it continues throughout the gastrulation. Zippering movement, with forming Brachet's cleft, gradually closes the gap to establish the contact between them. Subduction makes a trench between the anterior organizer and the prospective neuroectoderm, and the tissues face each other via the trench. During the early step of gastrulation, the anterior organizer moves to establish the physical contact with the prospective neuroectoderm through the 'subduction and zippering' movements. The organizer located at the blastocoel floor contributes to the anterior axial mesoderm including the prechordal plate, and the organizer at the dorsal lip ends up as the posterior axial mesoderm. In the model, the organizer is present at the blastocoel roof of blastulae, moves vegetally to locate at the region that lies from the blastocoel floor to the dorsal lip at the onset of gastrulation. Here we propose a unified model of amphibian gastrulation movement.
However, we found that this physical contact was already established at the equatorial region of very early gastrula in a wide variety of amphibian species. The organizer is believed to involute inward and migrate animally to make physical contact with the prospective head neuroectoderm at the blastocoel roof of mid- to late-gastrula. The dorsal blastopore lip (known as the Spemann organizer) is important for making the body plan in amphibian gastrulation.
